8OTK
Structure of ClpC Q11P N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.621, 84.621, 32.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.310 - 1.150 |
| R-factor | 0.1461 |
| Rwork | 0.145 |
| R-free | 0.16620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.076 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.310 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 45932 | 2233 |
| <I/σ(I)> | 14.9 | 1.3 |
| Completeness [%] | 97.7 | 96.3 |
| Redundancy | 19.5 | 16.5 |
| CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289.15 | 0.2M lithium sulfate, 0.1M sodium acetate pH 4.5, 50% w/v PEG 400 |
