8OT7
Crystal structure of an 8-repeat consensus TPR superhelix with Barium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 2.36296 |
Spacegroup name | P 32 |
Unit cell lengths | 70.237, 70.237, 73.568 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 60.830 - 3.300 |
R-factor | 0.19314 |
Rwork | 0.187 |
R-free | 0.29877 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.516 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.560 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.159 | 0.831 |
Rmeas | 0.185 | 0.946 |
Rpim | 0.063 | 0.336 |
Total number of observations | 49097 | 6877 |
Number of reflections | 6121 | 898 |
<I/σ(I)> | 5.5 | 2 |
Completeness [%] | 100.0 | |
Redundancy | 8 | 7.7 |
CC(1/2) | 0.962 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 20mM BaCl2 100 mM NaOAc, pH 5.5 30% MPD |