8OT7
Crystal structure of an 8-repeat consensus TPR superhelix with Barium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 2.36296 |
| Spacegroup name | P 32 |
| Unit cell lengths | 70.237, 70.237, 73.568 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.830 - 3.300 |
| R-factor | 0.19314 |
| Rwork | 0.187 |
| R-free | 0.29877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.516 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.560 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.159 | 0.831 |
| Rmeas | 0.185 | 0.946 |
| Rpim | 0.063 | 0.336 |
| Total number of observations | 49097 | 6877 |
| Number of reflections | 6121 | 898 |
| <I/σ(I)> | 5.5 | 2 |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | 7.7 |
| CC(1/2) | 0.962 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 20mM BaCl2 100 mM NaOAc, pH 5.5 30% MPD |
