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8OT7

Crystal structure of an 8-repeat consensus TPR superhelix with Barium

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALBA BEAMLINE XALOC
Synchrotron siteALBA
BeamlineXALOC
Temperature [K]100
Detector technologyPIXEL
Collection date2020-10-16
DetectorDECTRIS PILATUS 6M
Wavelength(s)2.36296
Spacegroup nameP 32
Unit cell lengths70.237, 70.237, 73.568
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution60.830 - 3.300
R-factor0.19314
Rwork0.187
R-free0.29877
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle1.516
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0258)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]73.5603.480
High resolution limit [Å]3.3003.300
Rmerge0.1590.831
Rmeas0.1850.946
Rpim0.0630.336
Total number of observations490976877
Number of reflections6121898
<I/σ(I)>5.52
Completeness [%]100.0
Redundancy87.7
CC(1/2)0.9620.567
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.529220mM BaCl2 100 mM NaOAc, pH 5.5 30% MPD

223790

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