8OS2

Structure of the human LYVE-1 (lymphatic vessel endothelial receptor-1) hyaluronan binding domain in an unliganded state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 41 21 2
Unit cell lengths	70.810, 70.810, 75.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.770 - 1.640
R-factor	0.1845
Rwork	0.183
R-free	0.22280
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.969
Data scaling software	xia2
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.770	1.670
High resolution limit [Å]	1.640	1.640
Rmeas	0.059	1.951
Number of reflections	303577	1181
<I/σ(I)>	21	1.2
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.5M NaCl, 0.1M Sodium/Potassium Phosphate pH 5.5