8OS1
X-ray structure of the Peroxisomal Targeting Signal 1 (PTS1) of Trypanosoma Cruzi PEX5 in complex with the PTS1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.331, 69.936, 71.106 |
Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
Resolution | 48.220 - 2.100 |
R-factor | 0.19544 |
Rwork | 0.193 |
R-free | 0.25284 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.429 |
Data reduction software | XDS |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 35080 | 2248 |
<I/σ(I)> | 3.7 | |
Completeness [%] | 95.9 | |
Redundancy | 2.8 | |
CC(1/2) | 0.959 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M MgCl2, 0.1 M Tris pH 8.5 and 30% (w/v) PEG 4000 |