8OR7
Structure of a far-red induced allophycocyanin from Chroococcidiopsis thermalis sp. PCC 7203
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-06 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 63 |
| Unit cell lengths | 130.540, 130.540, 106.496 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.520 - 2.800 |
| R-factor | 0.18093 |
| Rwork | 0.178 |
| R-free | 0.24016 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.511 |
| Data reduction software | xia2 |
| Data scaling software | pointless |
| Phasing software | ARP/wARP (ARP/wARP 8.0) |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.520 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.091 | 1.217 |
| Number of reflections | 32743 | 4760 |
| <I/σ(I)> | 20.3 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.5 | 16.9 |
| CC(1/2) | 1.000 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 289 | 0.5 M ammonium sulfate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 1.0 M lithium sulfate monohydrate |
