8OQK

Crystal structure of Tannerella forsythia sugar kinase K1058

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-05
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	131.940, 131.940, 89.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.216 - 2.000
Rwork	0.177
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x7f
RMSD bond length	0.007
RMSD bond angle	1.464
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.216	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	26844	1946
<I/σ(I)>	19.58
Completeness [%]	100.0
Redundancy	26.63
CC(1/2)	0.999	0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium cacodylate, MPD, PEG 8000