8OQH

Structure of the cytosolic domain of lysosome-associated TMEM55B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	29.412, 84.445, 32.177
Unit cell angles	90.00, 98.32, 90.00

Refinement procedure

Resolution	42.220 - 1.760
R-factor	0.1957
Rwork	0.194
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.361
Data reduction software	XDS (20220110)
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.220	1.823
High resolution limit [Å]	1.760	1.760
Rmerge	0.114	1.378
Rmeas	0.133
Rpim	0.067	0.800
Number of reflections	15173	1517
<I/σ(I)>	6.67
Completeness [%]	98.1
Redundancy	3.8
CC(1/2)	0.994	0.493

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	0.1M Tris pH8.5, 0.2M Sodium acetate trihydrate, 30% w/v PEG 4000