8OQE

Dirhodium tetraacetate/ribonuclease A adduct in the P3221 space group (6 h soaking)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	93
Detector technology	PIXEL
Collection date	2022-09-01
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5406
Spacegroup name	P 32 2 1
Unit cell lengths	66.795, 66.795, 64.904
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.601 - 1.500
Rwork	0.178
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	3.359
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.860	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.079	2.022
Rpim	0.028	0.975
Number of reflections	27269	1349
<I/σ(I)>	20	0.8
Completeness [%]	100.0	99.8
Redundancy	8.5	5.1
CC(1/2)	0.999	0.223

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	2.5 M NaCl 3.3 M HCOONa 0.1 M Acetate buffer