8OQD
Dirhodium tetraacetate/ribonuclease A adduct in the P3221 space group (1 h soaking)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU PhotonJet-R |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2022-08-24 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.5406 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 67.142, 67.142, 64.791 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.637 - 1.540 |
Rwork | 0.195 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 3.198 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.980 | 1.560 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.077 | 2.388 |
Rpim | 0.041 | 1.763 |
Number of reflections | 25583 | 1242 |
<I/σ(I)> | 12.3 | 0.4 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 4.1 | 2.6 |
CC(1/2) | 0.998 | 0.087 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 2.5 M NaCl 3.3 M HCOONa 0.1 M Acetate buffer |