8OQ0
Crystal structure of tailspike depolymerase (APK09_gp48) from Acinetobacter phage APK09
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.7749 |
Spacegroup name | H 3 2 |
Unit cell lengths | 88.688, 88.688, 421.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.150 - 2.590 |
R-factor | 0.19312 |
Rwork | 0.189 |
R-free | 0.27157 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 2.379 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.150 | 2.700 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.339 | 2.467 |
Rmeas | 0.349 | 2.566 |
Rpim | 0.081 | 0.674 |
Total number of observations | 377770 | 31386 |
Number of reflections | 20409 | 2302 |
<I/σ(I)> | 10.9 | 1.7 |
Completeness [%] | 99.1 | |
Redundancy | 18.5 | 13.6 |
CC(1/2) | 0.993 | 0.303 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 2% PEG200, 100mM HEPES pH 7.5, 20% tacsimate pH 7.0 |