8OPM

Human Coronavirus HKU1 spike glycoprotein in complex with an alpha2,8-linked 9-O-acetylated disialoside (closed state)

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.7
Number of Particle	956697
Software	cryoSPARC,PHENIX,Coot,EPU,UCSF Chimera,UCSF ChimeraX
Symmetry	C3
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION