8OPI
VHH Z70 mutant 1 in interaction with PHF6 Tau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978565 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 135.165, 135.165, 35.411 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.240 - 1.830 |
Rwork | 0.198 |
R-free | 0.23010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.827 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.240 | 44.240 | 1.870 |
High resolution limit [Å] | 1.830 | 8.970 | 1.830 |
Rmerge | 0.155 | 0.045 | 3.123 |
Rmeas | 0.159 | 0.046 | 3.200 |
Rpim | 0.034 | 0.010 | 0.700 |
Number of reflections | 17371 | 17371 | 191 |
<I/σ(I)> | 20 | ||
Completeness [%] | 100.0 | ||
Redundancy | 39.5 | 30.3 | 39.3 |
CC(1/2) | 1.000 | 0.999 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 1.7M Ammonium Sulfate, 4.25% Isopropanol, 15% lLycerol |