8OP0
VHH Z70 mutant 20 in interaction with PHF6 Tau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 135.309, 135.309, 35.508 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.230 - 1.540 |
Rwork | 0.160 |
R-free | 0.19670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.575 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.290 | 44.290 | 1.570 |
High resolution limit [Å] | 1.540 | 8.430 | 1.540 |
Rmerge | 0.071 | 0.057 | 1.788 |
Rmeas | 0.072 | 0.058 | 1.830 |
Rpim | 0.015 | 0.013 | 0.385 |
Number of reflections | 28067 | 191 | 1055 |
<I/σ(I)> | 31.5 | ||
Completeness [%] | 97.5 | ||
Redundancy | 40.8 | 31.2 | 42.8 |
CC(1/2) | 0.999 | 0.995 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.095M tri-Sodium citrate pH 5.6, 19% isopropanol, 15% Glycerol |