8OOZ
Glutamine synthetase from Methermicoccus shengliensis in complex with MgATP at 2.7 A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-27 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.548, 197.399, 135.171 |
Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.700 |
R-factor | 0.1972 |
Rwork | 0.196 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.348 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.845 | 2.878 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.062 | 0.688 |
Rmeas | 0.070 | 0.784 |
Rpim | 0.033 | 0.370 |
Number of reflections | 128572 | 6429 |
<I/σ(I)> | 17.4 | 2 |
Completeness [%] | 88.1 | 89.8 |
Redundancy | 4.3 | 4.4 |
CC(1/2) | 0.999 | 0.701 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293.15 | The protein was crystallized fresh without any freezing step, and obtained through the sitting drop method on a 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI, United Kingdom) under anaerobic conditions (N2:H2, gas ratio of 97:3). Crystallization was performed at 9 mg/ml glutamine synthetase in 25 mM Tris/HCl pH 7.6, 10% v/v glycerol, and 2 mM dithiothreitol. The reservoir contained 90 ul precipitant (1.6 M sodium citrate tribasic dihydrate). 0.55 uL protein was mixed with 0.55 uL precipitant. Crystals were soaked for 4 min in the crystallization solution supplemented with 10 mM ATP/Mg2+/sodium glutamate and then back soaked in the crystallization solution supplemented with 25% glycerol prior to freezing in liquid nitrogen. |