8OOX
Glutamine synthetase from Methermicoccus shengliensis at a resolution of 3.09 A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-17 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00002 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 226.465, 226.465, 226.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.370 - 3.090 |
R-factor | 0.1878 |
Rwork | 0.186 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.678 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 130.750 | 3.172 |
High resolution limit [Å] | 3.088 | 3.088 |
Rmerge | 0.345 | 3.099 |
Rmeas | 0.352 | 3.159 |
Rpim | 0.068 | 0.607 |
Number of reflections | 35711 | 1793 |
<I/σ(I)> | 12.2 | 1.4 |
Completeness [%] | 96.6 | 63.8 |
Redundancy | 26.6 | 26.8 |
CC(1/2) | 0.998 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293.15 | The protein was crystallized fresh without any freezing step, and obtained through the sitting drop method on a 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI, United Kingdom) under anaerobic conditions (N2:H2, gas ratio of 97:3). Crystallization was performed at 9 mg/ml glutamine synthetase in 25 mM Tris/HCl pH 7.6, 10% glycerol, and 2 mM dithiothreitol. The reservoir contained 90 ul precipitant (1.6 M sodium citrate tribasic dihydrate). 0.55 uL protein was mixed with 0.55 uL precipitant. Crystals were soaked in the precipitant supplemented with 30% v/v glycerol before freezing in liquid nitrogen. |