8OOM
Structural and functional studies of geldanamycin amide synthase ShGdmF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.874, 96.184, 86.754 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.270 - 1.820 |
R-factor | 0.1716 |
Rwork | 0.170 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.323 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.270 | 1.885 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.086 | 0.765 |
Number of reflections | 27488 | 2723 |
<I/σ(I)> | 13.04 | 2.11 |
Completeness [%] | 99.3 | |
Redundancy | 13.04 | |
CC(1/2) | 0.998 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 291 | 100 mM HEPES, pH: 7.5, 25% PEG4000, 150 mM sodium acide, 200 mM lithium sulfate |