8OO4
X-ray structure of the adduct formed upon reaction of cisplatin with human angiogenin after 1 month soaking
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.250, 37.510, 38.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.625 - 1.990 |
| Rwork | 0.308 |
| R-free | 0.36720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.526 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.630 | 2.020 |
| High resolution limit [Å] | 1.769 | 1.990 |
| Rmerge | 0.082 | 0.566 |
| Number of reflections | 10455 | 414 |
| <I/σ(I)> | 17.1 | 2.4 |
| Completeness [%] | 95.3 | 98.1 |
| Redundancy | 10 | 5.1 |
| CC(1/2) | 0.996 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG6K, 20 mM sodium citrate pH 5.1, 0.2 M sodium/potassium tartrate |
