8OO2
ChdA complex with amido-chelocardin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.779 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.580, 82.780, 99.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.780 - 1.850 |
R-factor | 0.1761 |
Rwork | 0.174 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 0.927 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASES |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.780 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.058 | 0.322 |
Rmeas | 0.062 | |
Number of reflections | 32227 | 2213 |
<I/σ(I)> | 24.5 | |
Completeness [%] | 99.5 | |
Redundancy | 11.8 | |
CC(1/2) | 0.999 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 16% (w/v) PEG 8000, 0.2 M sodium acetate and 0.1 M sodium cacodylate pH 6.5 |