8ONX

High resolution structure of Chaetomium thermophilum MAP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	277
Detector technology	PIXEL
Collection date	1021-09-04
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9737
Spacegroup name	P 21 21 21
Unit cell lengths	59.938, 73.806, 82.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.020 - 1.300
R-factor	0.1619
Rwork	0.161
R-free	0.17680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.530	1.320
High resolution limit [Å]	1.300	1.300
Rpim	0.015	0.288
Number of reflections	90066	4362
<I/σ(I)>	23
Completeness [%]	99.9
Redundancy	12.3
CC(1/2)	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350