8ONW
Crystal structure of the hetero-dimeric complex from Archaeoglobus fulgidus PRC1 and PRC2 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.086, 102.633, 112.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.020 - 2.290 |
Rwork | 0.230 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.036 |
Data reduction software | DIALS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | REFMAC (v5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.020 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmeas | 0.048 | |
Number of reflections | 30556 | 463 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 89.0 | |
Redundancy | 6 | |
CC(1/2) | 0.500 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% MPD, 40 mM magnesium chloride, 50 mM sodium cacodylate pH 6 |