8ONI
Human insulin in complex with the analytical antibody S1 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.307, 71.896, 99.640 |
Unit cell angles | 90.00, 112.39, 90.00 |
Refinement procedure
Resolution | 41.620 - 2.300 |
R-factor | 0.1995 |
Rwork | 0.197 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 0.732 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.620 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.062 | 0.201 |
Rmeas | 0.074 | 0.244 |
Rpim | 0.040 | 0.136 |
Number of reflections | 48254 | 13829 |
<I/σ(I)> | 19.81 | 6.41 |
Completeness [%] | 99.2 | 95.74 |
Redundancy | 3.4 | 3 |
CC(1/2) | 0.996 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 25%(w/v) PEG 2000 MME |