8OMG
Crystal structure of mKHK (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 6 |
| Unit cell lengths | 154.390, 154.390, 50.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.850 - 1.820 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.850 | 2.094 |
| High resolution limit [Å] | 1.820 | 2.087 |
| Rmerge | 0.095 | 0.981 |
| Number of reflections | 40725 | 39776 |
| <I/σ(I)> | 15.3 | 2.3 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 10.3 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 14% PEG 8000, 0.2 M ammonium sulfate, 0.1 M tri-sodium citrate pH 4.9 |
