8OMC
Y345F/F347Y/Y389F Variant of Dye Type Peroxidase Aa (DtpAa) from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.852, 67.930, 74.483 |
| Unit cell angles | 90.00, 105.46, 90.00 |
Refinement procedure
| Resolution | 58.260 - 1.500 |
| R-factor | 0.1622 |
| Rwork | 0.161 |
| R-free | 0.18922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.005 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.260 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.078 | 1.336 |
| Rmeas | 0.092 | 1.582 |
| Rpim | 0.049 | 0.840 |
| Number of reflections | 110533 | 5452 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.999 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1:1 RATIO OF A 15 MG/ML PROTEIN SOLUTION WITH A PRECIPITANT SOLUTION CONTAINING 20% PEG 6000, 100 MM HEPES PH 7.0 |
