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8OLY

Structure of Oceanobacillus iheyensis group II intron post first step of splicing in the presence of K+, Mg2+ and intronistat B

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE MASSIF-1
Synchrotron siteESRF
BeamlineMASSIF-1
Temperature [K]100
Detector technologyPIXEL
Collection date2021-11-15
DetectorDECTRIS PILATUS 2M
Wavelength(s)0.96
Spacegroup nameP 21 21 21
Unit cell lengths88.518, 94.686, 222.874
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.020 - 3.110
R-factor0.20146
Rwork0.200
R-free0.23712
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.598
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.0203.200
High resolution limit [Å]3.1003.100
Number of reflections341013245
<I/σ(I)>10.57
Completeness [%]98.7
Redundancy4.2
CC(1/2)1.0000.220
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP303.15100 mM magnesium acetate, 100 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3.5% PEG8000

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