8OLW
Structure of Oceanobacillus iheyensis group II intron before the first step of splicing in the presence of K+, Ca2+ and intronistat B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.147, 94.133, 222.878 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.680 - 4.000 |
R-factor | 0.22225 |
Rwork | 0.220 |
R-free | 0.25804 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.826 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.680 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.097 | |
Number of reflections | 16065 | 1442 |
<I/σ(I)> | 5.8 | |
Completeness [%] | 97.8 | |
Redundancy | 4.6 | |
CC(1/2) | 1.000 | 0.280 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM potassium acetate, 100 mM potassium chloride, 100 mM calcium chloride, 50 mM HEPES sodium, pH 7.0, 4.5% PEG8000 |