8OKW
Crystal structure of Bdellovibrio bacteriovorus Bd2734 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.161, 87.682, 52.754 |
| Unit cell angles | 90.00, 118.88, 90.00 |
Refinement procedure
| Resolution | 45.170 - 1.840 |
| R-factor | 0.141 |
| Rwork | 0.139 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.195 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 2.010 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 23686 | 1184 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 65.1 | |
| Redundancy | 3 | |
| CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M ammonium citrate dibasic, 20 % w/v PEG 3350 |
