8OK7

Variant Surface Glycoprotein VSG558 NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	103.080, 64.120, 155.040
Unit cell angles	90.00, 109.13, 90.00

Refinement procedure

Resolution	53.550 - 1.740
R-factor	0.239
Rwork	0.238
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.921
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.550	1.800
High resolution limit [Å]	1.740	1.740
Number of reflections	196293	19469
<I/σ(I)>	5.36
Completeness [%]	98.7
Redundancy	6
CC(1/2)	0.996	0.268

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	100 mM Ada/NaOH 20 % (w/v) PEG 1500 200 mM KCl