8OK4
Variant Surface Glycoprotein VSG11wt-Oil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.862, 74.862, 105.611 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.940 - 1.230 |
| R-factor | 0.1631 |
| Rwork | 0.162 |
| R-free | 0.18840 |
| Structure solution method | SAD |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.940 | 1.274 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.075 | 1.777 |
| Number of reflections | 99764 | 9827 |
| <I/σ(I)> | 15.79 | 0.98 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.4 | |
| CC(1/2) | 1.000 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 1.7 M NaKTartrate, 100 mM Tris/HCl |
