8OK3
Structure of the C-terminal domain of the Bdellovibrio bacteriovorus Bd2133 fibre
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 3 |
Unit cell lengths | 68.760, 68.760, 68.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.750 - 1.500 |
R-factor | 0.1508 |
Rwork | 0.150 |
R-free | 0.15920 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.750 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 17441 | 1266 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.1 | 98 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.996 | 0.815 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.03M magnesium chloride, 0.03M calcium chloride, 0.1 M imidazole/MES pH 6.5, 12.5 % v/v MPD, 12.5 % PEG 1000, 12.5 % w/v PEG 3350 |