8OJU
Crystal structure of the human IgD Fab - structure Fab3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-29 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.893, 74.198, 70.915 |
Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
Resolution | 51.250 - 1.450 |
R-factor | 0.1585 |
Rwork | 0.157 |
R-free | 0.18930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.932 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.870 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.077 | |
Rpim | 0.032 | 0.661 |
Number of reflections | 78636 | 3890 |
<I/σ(I)> | 11.2 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 5.1 |
CC(1/2) | 0.999 | 0.462 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 16% PEG 6000 and 0.1M tri-sodium citrate |