8OJT
Crystal structure of the human IgD Fab - structure Fab2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-29 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9179 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.149, 73.827, 70.458 |
Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
Resolution | 70.390 - 1.550 |
R-factor | 0.1623 |
Rwork | 0.161 |
R-free | 0.19760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.390 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.099 | |
Rpim | 0.040 | 0.923 |
Number of reflections | 64083 | 3165 |
<I/σ(I)> | 10 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 6.2 |
CC(1/2) | 0.998 | 0.486 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M BTP pH 6.5, 20% PEG 3350 and 0.2M sodium nitrate |