8OJF

Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 with bound phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.981
Spacegroup name	P 21 21 2
Unit cell lengths	127.220, 158.170, 141.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.706 - 3.037
R-factor	0.20485384429
Rwork	0.203
R-free	0.24268
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.878
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	3.220
High resolution limit [Å]	3.029	3.029
Rmerge	0.152	2.630
Number of reflections	55586	8756
<I/σ(I)>	10.86
Completeness [%]	99.8	89.9
Redundancy	13.74	13.96
CC(1/2)	0.999	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.09M Sodium nitrate, 0.09 Sodium phosphate dibasic, 0.09M Ammonium sulfate, 0.1M MES monohydrate pH 6.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8K