8OIY
Crystal structure of the R15908H missense variant of titin domain Fn3-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 52.480, 56.030, 56.090 |
Unit cell angles | 60.06, 70.16, 65.62 |
Refinement procedure
Resolution | 46.340 - 2.100 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.110 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.116 | 0.324 |
Number of reflections | 25781 | 2523 |
<I/σ(I)> | 5.36 | |
Completeness [%] | 88.9 | |
Redundancy | 1.9 | |
CC(1/2) | 0.993 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10mM Tris-HCl PH 7.5 NaCl 0.5mM DTT 0.1M sodium acetate pH 4.5 0.2M Lithium sulphate 50% v/v PEG 400 |