8OIG
Crystal Structure of Staphopain C from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 31 |
| Unit cell lengths | 103.136, 103.136, 51.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.570 - 1.580 |
| R-factor | 0.08824 |
| Rwork | 0.088 |
| R-free | 0.11597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.916 |
| Data reduction software | AutoProcess |
| Data scaling software | AutoProcess |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.570 | 1.639 |
| High resolution limit [Å] | 1.580 | 1.582 |
| Rmerge | 0.054 | |
| Number of reflections | 80816 | 8329 |
| <I/σ(I)> | 11.07 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M AmSO4, 0.1M Tris, ph 8.5, 12% glycerol |
