8OIG
Crystal Structure of Staphopain C from Staphylococcus aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-14 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.05 |
Spacegroup name | P 31 |
Unit cell lengths | 103.136, 103.136, 51.204 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.570 - 1.580 |
R-factor | 0.08824 |
Rwork | 0.088 |
R-free | 0.11597 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.916 |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.570 | 1.639 |
High resolution limit [Å] | 1.580 | 1.582 |
Rmerge | 0.054 | |
Number of reflections | 80816 | 8329 |
<I/σ(I)> | 11.07 | |
Completeness [%] | 97.6 | |
Redundancy | 3.8 | |
CC(1/2) | 0.997 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.5 M AmSO4, 0.1M Tris, ph 8.5, 12% glycerol |