8OIC
Trichomonas vaginalis riboside hydrolase (His-tagged)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-23 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 73.055, 94.665, 120.589 |
Unit cell angles | 105.08, 89.96, 93.83 |
Refinement procedure
Resolution | 116.418 - 2.800 |
Rwork | 0.209 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.205 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.420 | 2.623 |
High resolution limit [Å] | 2.578 | 2.578 |
Rpim | 0.089 | 0.855 |
Number of reflections | 96699 | 4868 |
<I/σ(I)> | 6.3 | 0.9 |
Completeness [%] | 98.5 | 98.2 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.993 | 0.361 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M bicine pH 8.5, 20% (w/v) PEG 5000 monomethyl ether, 3% sucrose |