8OIC
Trichomonas vaginalis riboside hydrolase (His-tagged)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-23 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.055, 94.665, 120.589 |
| Unit cell angles | 105.08, 89.96, 93.83 |
Refinement procedure
| Resolution | 116.418 - 2.800 |
| Rwork | 0.209 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.205 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.420 | 2.623 |
| High resolution limit [Å] | 2.578 | 2.578 |
| Rpim | 0.089 | 0.855 |
| Number of reflections | 96699 | 4868 |
| <I/σ(I)> | 6.3 | 0.9 |
| Completeness [%] | 98.5 | 98.2 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.993 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.1 M bicine pH 8.5, 20% (w/v) PEG 5000 monomethyl ether, 3% sucrose |
