8OIB
Trichomonas vaginalis riboside hydrolase in complex with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-22 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.034, 156.998, 88.729 |
Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
Resolution | 88.725 - 2.440 |
Rwork | 0.189 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.179 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.730 | 2.480 |
High resolution limit [Å] | 2.440 | 2.440 |
Rpim | 0.058 | 0.317 |
Number of reflections | 50817 | 2518 |
<I/σ(I)> | 6.1 | 2.1 |
Completeness [%] | 98.0 | 99.2 |
Redundancy | 3 | 3.1 |
CC(1/2) | 0.993 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.85 | 291.15 | 200 mM ammonium citrate dibasic pH 4.85, 15% w/v PEG 5000 monomethyl ether, 17.5% (v/v) glycerol |