8OI4
Metagenomic Beta-galactosidase from Glycoside Hydrolase family GH154
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.316, 94.581, 108.264 |
| Unit cell angles | 90.00, 105.45, 90.00 |
Refinement procedure
| Resolution | 47.400 - 1.760 |
| R-factor | 0.15325 |
| Rwork | 0.152 |
| R-free | 0.17110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 177698 | 5125 |
| <I/σ(I)> | 6.1 | 0.8 |
| Completeness [%] | 96.0 | 56.1 |
| Redundancy | 2.53 | |
| CC(1/2) | 0.987 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 16% (w/v) PEG 3350, 100 mM HEPES-NaOH, pH 7.5, 150 mM NaCl |
