8OI2
Crystal structure of Alb1 megabody in complex with human serum albumin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 370.359, 73.819, 66.130 |
Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
Resolution | 33.860 - 3.300 |
R-factor | 0.2279 |
Rwork | 0.226 |
R-free | 0.27670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.609 |
Data reduction software | AutoProcess |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.270 | 3.530 |
High resolution limit [Å] | 3.250 | 3.300 |
Rmerge | 0.044 | 0.937 |
Rpim | 0.044 | 0.932 |
Number of reflections | 43671 | 4847 |
<I/σ(I)> | 11 | 1.1 |
Completeness [%] | 98.4 | 99.6 |
Redundancy | 3 | 3 |
CC(1/2) | 0.998 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 293 | 0.03M Sodium fluoride, 0.03M sodium bromide, 0.03M sodium iodide, 0.1M Tris BICINE pH 8.5, 12% v/v ethylene glycol, 6% w/v PEG 8000 |