8OGC

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.740, 81.740, 234.101
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.889 - 2.090
Rwork	0.171
R-free	0.20150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.211
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	234.100	2.150
High resolution limit [Å]	2.090	2.090
Number of reflections	28474	2159
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	19
CC(1/2)	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Tris pH 7.5, 2.18M Lithium acetate