8OG8

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.811, 81.811, 232.957
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.031 - 2.110
Rwork	0.174
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.150
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.590	2.170
High resolution limit [Å]	2.110	2.110
Number of reflections	27598	2186
<I/σ(I)>	14.4
Completeness [%]	100.0
Redundancy	14.3
CC(1/2)	0.998	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M MES pH6.5, 2.13M Lithium acetate