8OFV
Human adenovirus type 53 fiber-knob protein complexed with sialic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.91188 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 93.555, 60.406, 243.686 |
Unit cell angles | 90.00, 99.88, 90.00 |
Refinement procedure
Resolution | 92.170 - 1.770 |
R-factor | 0.20937 |
Rwork | 0.208 |
R-free | 0.22826 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.537 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.170 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.168 | 1.707 |
Rmeas | 0.195 | 1.967 |
Rpim | 0.097 | 0.966 |
Number of reflections | 258349 | 12686 |
<I/σ(I)> | 6 | 0.6 |
Completeness [%] | 98.7 | 97.7 |
Redundancy | 3.8 | 3.9 |
CC(1/2) | 0.685 | 0.327 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 0.1 M PCTP buffer, 25 % w/v PEG 1500 |