8OFT
Human adenovirus type 32 fiber-knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91188 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.946, 93.648, 94.840 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 93.810 - 2.000 |
| R-factor | 0.21994 |
| Rwork | 0.218 |
| R-free | 0.25641 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.489 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.810 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.102 | 0.551 |
| Rmeas | 0.119 | 0.658 |
| Rpim | 0.062 | 0.353 |
| Number of reflections | 39152 | 2762 |
| <I/σ(I)> | 5.9 | 1.4 |
| Completeness [%] | 99.4 | 94.8 |
| Redundancy | 3.6 | 3.3 |
| CC(1/2) | 0.992 | 0.831 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M SPG Buffer, 25% PEG 1500 |
