8OFS

Human adenovirus type 30 fiber-knob protein complexed with sialic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	62.130, 62.490, 71.800
Unit cell angles	90.00, 93.85, 90.00

Refinement procedure

Resolution	48.560 - 2.560
R-factor	0.2319
Rwork	0.229
R-free	0.29189
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.222
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.490	2.630
High resolution limit [Å]	2.560	2.560
Rmerge	0.057	0.972
Rmeas	0.066	1.131
Rpim	0.034	0.570
Total number of observations		5020
Number of reflections	17735	1327
<I/σ(I)>	10.5	1.1
Completeness [%]	99.0
Redundancy	3.7	3.8
CC(1/2)	0.998	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.1M MMT [Malic acid, MES, Tris], 25% w/v PEG 1500