8OFJ

Mycoplasma pneumoniae CdaM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 1 21 1
Unit cell lengths	38.840, 80.420, 44.444
Unit cell angles	90.00, 93.13, 90.00

Refinement procedure

Resolution	38.854 - 2.250
Rwork	0.198
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.085
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.060	2.310
High resolution limit [Å]	2.250	7.120	2.250
Rmerge	0.122	0.040	1.308
Rmeas	0.132	0.044	1.412
Number of reflections	12934	277	958
<I/σ(I)>	11.68
Completeness [%]	99.3
Redundancy	6.9
CC(1/2)	0.998	0.999	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% (v/v) PEG MME 2000 0.1M Na-HEPES pH 7.5