8OFB
Crystal Structure of T. maritima reverse gyrase with a minimal latch, hexagonal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-03 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 90.833, 90.833, 571.878 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.850 - 2.390 |
R-factor | 0.2447 |
Rwork | 0.243 |
R-free | 0.27240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.9) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 78.660 | 78.660 | 2.590 |
High resolution limit [Å] | 2.390 | 7.440 | 2.390 |
Rmerge | 0.293 | 0.072 | 1.986 |
Rmeas | 0.301 | 0.074 | 2.037 |
Rpim | 0.069 | 0.018 | 0.449 |
Number of reflections | 45491 | 2274 | 2276 |
<I/σ(I)> | 7.5 | 18.1 | |
Completeness [%] | 94.8 | 99.9 | 67.4 |
Redundancy | 18.7 | 15.5 | 20.3 |
CC(1/2) | 0.997 | 0.999 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 54 microM protein in 50mM Tris/HCl, pH7.5, 500mM NaCl, 10mM MgCl2, 100 microM ZnCl2, 2mM BME mixed 1:1 with 0.1M Tris/HCl pH7.5, 19% PEG3350 0.29 M Ammonium Sulfate, total volume 400nL |