8OF3
Structure of the apoform of ALDEHYDE DEHYDROGENASE 5F1 (ALDH5F1) from the moss Physcomitrium patens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-26 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.99187 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 212.689, 212.689, 186.742 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 131.140 - 1.348 |
| R-factor | 0.1494 |
| Rwork | 0.149 |
| R-free | 0.16410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.180 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.140 | 1.500 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.156 | 1.800 |
| Number of reflections | 241670 | 12083 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 68.0 | |
| Redundancy | 20 | |
| CC(1/2) | 0.998 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 292 | 1.7M LiSO4 |
