8ODQ
SufS-SufU complex from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.250, 75.553, 75.602 |
| Unit cell angles | 92.40, 109.57, 99.71 |
Refinement procedure
| Resolution | 48.720 - 1.650 |
| R-factor | 0.18056 |
| Rwork | 0.179 |
| R-free | 0.21373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.104 | |
| Number of reflections | 147290 | 22934 |
| <I/σ(I)> | 7.47 | 0.96 |
| Completeness [%] | 95.7 | 92.4 |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.996 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.4 M KNO3, 16% PEG 3350, 0.1M MOPS pH 6.7 |
