8KHW
The crystal structure of glycosaminoglycan lyase GAGase VII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.407, 98.407, 134.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.870 - 2.400 |
R-factor | 0.2122 |
Rwork | 0.207 |
R-free | 0.27140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.986 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.870 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.098 | 0.720 |
Rmeas | 0.100 | 0.737 |
Rpim | 0.020 | 0.158 |
Number of reflections | 49305 | 2737 |
<I/σ(I)> | 20.6 | 4.3 |
Completeness [%] | 99.7 | 99.85 |
Redundancy | 23.6 | 21.4 |
CC(1/2) | 0.999 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES (pH 7.0), 36% (v/v) PEG 400 |