8KHO

Crystal structure of human methionine aminopeptidase 12 (MAP12) in complex with two Cobalt ions and Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97957
Spacegroup name	C 1 2 1
Unit cell lengths	79.360, 81.070, 49.020
Unit cell angles	90.00, 102.32, 90.00

Refinement procedure

Resolution	28.020 - 1.450
R-factor	0.1842
Rwork	0.183
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.907
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.020	4.300	1.530
High resolution limit [Å]	1.450	3.060	1.450
Rmerge	0.071	0.046	0.548
Rmeas	0.083	0.054	0.638
Number of reflections	53554	3589	8613
<I/σ(I)>	10.4
Completeness [%]	99.2
Redundancy	3.7
CC(1/2)	0.997	0.996	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	0.1M HEPES pH 7.2, 0.1M Carboxylic acids, 17% (v/v) MPD, 17% (w/v) PEG 1000, 17% (w/v) PEG 3350