8KHN

Crystal structure of human methionine aminopeptidase 12 (MAP12) in complex with two cobalt ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97957
Spacegroup name	C 1 2 1
Unit cell lengths	78.916, 80.978, 49.033
Unit cell angles	90.00, 102.21, 90.00

Refinement procedure

Resolution	27.350 - 1.510
R-factor	0.1936
Rwork	0.193
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.350	27.350	1.600
High resolution limit [Å]	1.510	4.480	1.510
Rmerge	0.091	0.035	0.922
Rmeas	0.107	0.042	1.071
Number of reflections	46575	1826	7348
<I/σ(I)>	8.61
Completeness [%]	98.8
Redundancy	3.7
CC(1/2)	0.997	0.997	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1M HEPES pH 7.2, 0.1M Carboxylic acids, 17% (v/v) MPD, 17% (w/v) PEG 1000, 17% (w/v) PEG 3350